@conference {41648,
	title = {Identification of commensal bacterial metabolites that enhance the integrity of the gastrointestinal barrier},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {66},
	year = {2006},
	month = {Jan},
	pages = {225-229},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Napier},
	abstract = {The objective of this research was to identify metabolites produced by commensal bacteria that enhance the integrity of the gastrointestinal barrier (GIB). The integrity of the GIB is mainly determined by the complexity of interactions between tight junctions of opposing epithelial cells of the GI wall. This was measured using an in vitro bioassay, consisting of a monolayer of intestinal epithelial cells grown on a membrane. The trans-epithelial electrical resistance (TEER) across this monolayer was measured to determine the strength of the tight junctions. Preliminary results showed that a commercial probiotic product caused an increase in TEER across non-differentiated and differentiated monolayers by 178 {\textpm} 61\% and 40 {\textpm} 9\%, respectively. The maximum increases in TEER obtained with conditioned media fractions (media fractions containing components secreted by the probiotic product) was between 23 {\textpm} 6\% and 43 {\textpm} 4\%. The conditioned media fraction containing components less than 3 kDa accounted for the total activity of the whole conditioned media. Metabolite profiling of active conditioned media compared to inactive conditioned media using fast-LCMSMS identified numerous metabolites that may be responsible for this positive effect. Future work will isolate the candidate metabolites and screen them for GIB enhancing activity both in vitro and in vivo. },
	issn = {1176-5283},
	author = {Anderson RC and Cassidy LA and Cookson AL and Koulman A and Hurst RD and Fraser K and McNabb WC and Lane G and Roy NC}
}
@conference {41645,
	title = {Metabolomics for animal production : An introduction},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {66},
	year = {2006},
	month = {Jan},
	pages = {209-212},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Napier},
	abstract = {Metabolomics will be one of the most important tools in the exploitation of the bovine genome sequencing project. The results of metabolomics will play important roles in many facets of animal production. This paper will give an introduction of metabolomics and its application in the field of animal production. It serves as the start of the metabolomics session at the 2006 conference of New Zealand Society of Animal Production covering the origin, analytical chemistry and ongoing research in metabolomics with a special focus on this research within New Zealand. },
	issn = {1176-5283},
	author = {Koulman A and Roy NC}
}
@conference {41898,
	title = {Systems biology and metabolomics : Experimental and computational challenges},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {66},
	year = {2006},
	month = {Jan},
	pages = {213-218},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Napier},
	abstract = {High-throughput technologies in the era of post-genomics provide new opportunities to investigate complex biological systems. Data can be collected for a system of interest at the level of gene expression, protein expression and metabolite concentration changes and then used for the assessment of interactions of molecules. However, the vast amount of information also poses challenges for data management, analysis, and interpretation. This paper aims to outline the technical complexity and the sources of variation in the technologies involved in molecular systems biology and computational strategies for data integration. },
	issn = {1176-5283},
	author = {Cao M and Koulman A and Pacheco D and Lane GA and Rasmussen S}
}
@conference {41901,
	title = {Urinary biomarkers of forage feeding from ESI-MS/MS fingerprinting: exploratory studies},
	booktitle = {Proceedings of the New Zealand Society of Animal Production},
	volume = {66},
	year = {2006},
	month = {Jan},
	pages = {230-235},
	publisher = {New Zealand Society of Animal Production},
	organization = {New Zealand Society of Animal Production},
	address = {Napier},
	abstract = {The combination of metabolic profiling by a combination of broad-spectrum analytical methods and multivariate statistics offers new opportunities for obtaining a global over-view of metabolism. It also facilitates the discovery of metabolites which can serve as useful markers of biological processes or states ("biomarkers"). Fast liquid chromatography and electrospray ionisation mass spectrometry with collision-induced dissociation in an ion-trap (fast LC-ESI-MS/MS) has been applied to determine metabolic profiles of bovine urine samples. The samples were collected twice daily from groups of five lactating dairy cows grazing in four different forage management systems comprising different spatial and temporal allocations of ryegrass (Lolium perenne) and white clover (Trifolium repens). The data have been analysed by different multivariate statistical methods, and a number of candidate biomarkers of forage feed and diurnal processes have been identified. },
	issn = {1176-5283},
	author = {Lane GA and Fraser K and Cao M and Koulman A and Cosgrove GP and Death A}
}
